eukaryoticexpression相关论文
Puccinia striiformis west.f.sp.tritici (Pst) is the causal agent of wheat stripe rust which is one of the most serious d......
Jawless vertebrates represented by lampreys and hagfish use variable lymphocyte receptors (VLRs) as antigen receptors to......
Expression of Spodoptera litura innexin2 and innexin3 proteins in HeLa cells to explore their gap ju
Gap junction proteins include connexins (Cx) from vertebrate and innexins from invertebrate.Endogenous innexins extensiv......
Cinnamate 4-hydroxylase(C4H)was a key enzyme in the phenylpropanoid pathway in tea plant.The gene can influence the bios......
Herpers simplex virus (HSV) is one of the common viruses which can infect human, and it is prevalent all over the wo......
参考GenBank发表猪IL-15mRNA序列设计引物,用RT-PCR方法扩增猪IL-15cDNA,并克隆到pMD18-T载体中,通过PCR、酶切和测序验证克隆正确......
利用PCR方法扩增出鸡β-肌动蛋白(β-actin)启动子基因序列,克隆至pcDNA3.1和pCIneo载体中以替代CMV启动子,获得含鸡源启动子的真......
为验证水疱性口炎病毒（vesicularstomatitisvirus，VSV）糖蛋白与半乳糖凝集素1（1ectingalactoside—bindingsoluble1，LGALSl）之间的相互作......
利用聚合酶链式反应（PCR），以羊布鲁菌16M基因组为模板克隆Omp25基因，设计含有EcoRI和xhoI酶切位点的引物序列，定向克隆到真核表达载体pC......
为构建犬新孢子虫NcSRS9基因真核表达重组质粒,了解NcSRS9表面蛋白基因的生物学特性及其体外表达情况。本试验应用PCR技术获得目片......
目的：观察小鼠各脏器中Rab5a表达情况,并构建Rab5a真核表达载体并验证其表达情况。方法：采用RT-PCR和Real-time PCR方法检测Rab5a在......
Effect of antisense human telomerase RNA on malignant behaviors of gastric carcinoma cell line SGC-7
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Objective:To express two Livin isoforms (Livin α & β genes) with transfection techniques in A549 cell line respectivel......
Expression of TIMP-3 Gene by Construction of a Eukaryotic Cell Expression Vector and Its Role in Red
The present study is aimed at studying the gene for TIMP-3, a mammalian tissue inhibitor, by constructing a recombinant ......
目的 构建大鼠心脏kir2.2、kir2.3通道真核表达质粒,并鉴定其在人胚胎肾细胞(HEK293)中的表达.方法 提取大鼠心脏组织细胞RNA,逆转......
AIM: To seek for an effective method to improve the immuneresponses induced by DNA vaccine expressing HBV surfaceantigen......
AIM: To study the effects of sense and antisense KAI1genes on the growth and invasion of human hepatocellularcarcinoma (......
Inhibitor RNA blocks the protein translation mediated by hepatitis C virus internal ribosome entry s
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目的 构建刚地弓形虫PRU株ROP19蛋白的真核表达载体并检测其表达.方法 利用生物信息学方法分析弓形虫ROP19蛋白的理化性质并比较弓......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
